Generation of single guide RNAs by assembling oligos and in vitro RNA synthesis
Synthesis of gRNAs in multiplex
Zebrafish injections. If you are new to injections, watch the video associated with this manuscript to get an idea of the process.
Immunostaining to label central canal cilia alongside various counterstains.
Calcein staining of zebrafish larvae.
Cloning a tandem gRNA insect expression vector using pCFD4
Collecting and fixing embryos for ISH or IF
Making apple juice plates
Generation of DIG-labelled riboprobes from synthesized templates
RNA extraction from zebrafish embryos using columns (18-32 hpf)
RNA extraction from zebrafish embryos using TRIzol
In vitro mRNA synthesis from plasmid DNA
Quantitative PCR using ddCT method
This document describes some basic approaches to analyzing and plotting categorical data
